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    You are on page 1of 7 Search inside document Human Immunology xxx xxxxxx Contents lists available at ScienceDirect www. Association analyses were conducted on the whole data set. Introduction autoimmune disorders that were thought to be caused by complex interaction of multiple susceptibility genes and environmental fac- Rheumatoid arthritis RA [MIM ] and systemic lupus tors and affects approximately 0. Familiar and twins studies have provided robust evidence for the Corresponding author at: Department of Human Anatomy, Histology and role of genetic factors in these diseases [2,3]. Please cite this article in press as: L. Tang et al. For the RA patient cohorts, unrelated patients above hypothesis. However, non-MHC alleles were also required. Rheumatoid fac- Lyp , was reported to be significantly associated with multiple tor RF status was determined for all the patients. For SLE, a total of Graves disease [7]. The association Medical University. Subsequently, Begovich et al. In addition, observed a significant association between the same PTPN22 unrelated control subjects who without the history of multi- RW variant and rheumatoid arthritis RA [9]. Furthermore, ple autoimmune diseases matched for ethnicity, gender and age Kyogoku et al. All participants were Chinese Han in origin.

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    In addition, observed a significant association between the same PTPN22 unrelated control subjects who without the history of multi- RW variant and rheumatoid arthritis RA [9]. Furthermore, ple autoimmune diseases matched for ethnicity, gender and age Kyogoku et al. All participants were Chinese Han in origin. Genotyping susceptibility to both systemic and organ-specific autoimmune diseases.

    Genomic DNA was extracted from tions. Considering the important role of PTPN22 in the pathogene- peripheral leukocytes using the standard phenolchloroform sis of autoimmune diseases, more variants except for RW method. Cycling parameters were as follows: 95 "C for 2 min; 10 PTPN22 gene in autoimmune diseases in this population.

    Moreover, cycles at 95 "C for 20 s, 67 "C! In the present study, we aim selected at random to perform repeat assays as genotyping quality to ascertain whether the PTPN22 polymorphisms were etiologically control.

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