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In addition, observed a significant association between the same PTPN22 unrelated control subjects who without the history of multi- RW variant and rheumatoid arthritis RA . Furthermore, ple autoimmune diseases matched for ethnicity, gender and age Kyogoku et al. All participants were Chinese Han in origin. Genotyping susceptibility to both systemic and organ-specific autoimmune diseases.
Genomic DNA was extracted from tions. Considering the important role of PTPN22 in the pathogene- peripheral leukocytes using the standard phenolchloroform sis of autoimmune diseases, more variants except for RW method. Cycling parameters were as follows: 95 "C for 2 min; 10 PTPN22 gene in autoimmune diseases in this population.
Moreover, cycles at 95 "C for 20 s, 67 "C! In the present study, we aim selected at random to perform repeat assays as genotyping quality to ascertain whether the PTPN22 polymorphisms were etiologically control.